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. Author manuscript; available in PMC: 2012 Apr 19.
Published in final edited form as: Int J Oncol. 2011 Apr 21;39(1):111–119. doi: 10.3892/ijo.2011.1014

Figure 6.

Figure 6

Effect of TSA and valproic acid on TMPRSS2-ERG expression. (A) Western blot assay was performed after 12 or 24 h valproic acid treatments at the indicated concentrations. The blot with anti-ERG antibody is shown at the top. β-actin used as a loading control is shown at the bottom. (B) Western blot assay was performed after 12 or 24 h TSA treatments at the indicated concentrations. The blot with anti-ERG antibody is shown at the top. β-actin used as a loading control is shown at the bottom. (C) The relative expression level of TMPRSS2-ERG mRNA was determined with quantitative RT-PCR after 24 h treatment with 10 mM valproic acid. (D) The relative expression level of TMPRSS2-ERG mRNA was determined with quantitative RT-PCR after 24 h treatment with 1 μM TSA. In both cases, GAPDH was used to normalize samples. The results represent relative values against control (arbitrarily set to 1) of three independent experiments with standard deviations. * P<0.05

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