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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Curr Protoc Mol Biol. 2011 Jul;CHAPTER:Unit–17.6. doi: 10.1002/0471142727.mb1706s95

Table 1.

Antbodies and Lectins that Recognize O-GlcNAc and GlcNAc

Name Antibody Isotype Recognizes Commercially Available Positive Control Negative Control Citation
CTD110.6 IgM O-GlcNAc Covance, Pierce, SIGMA-Aldrich, Cell Signaling, Santa Cruz Biotechnlogy BSA-AP-GlcNAc BSA-AP or Ovalbumin (Comer et al. 2001)
RL2 IgG O-GlcNAc Abcam, Affinity Bioreagents, Santa Cruz Biotechnology BSA-AP- GlcNAc BSA-AP or Ovalbumin (Snow et al. 1987)
MY95 IgG O-GlcNAc ? BSA-AP- GlcNAc BSA-AP or Ovalbumin (Matsuoka et al. 2002)
18B10.C7(3) IgG O-GlcNAc Millipore BSA-AP- GlcNAc BSA-AP or Ovalbumin (Teo et al. 2010)
9D1.E4(10) IgG O-GlcNAc Millipore BSA-AP- GlcNAc BSA-AP or Ovalbumin (Teo et al. 2010)
1F5.D6(14) IgG O-GlcNAc Millipore BSA-AP- GlcNAc BSA-AP or Ovalbumin (Teo et al. 2010)
6D93 IgG O-GlcNAc Santa Cruz Biotechnology BSA-AP- GlcNAc BSA-AP or Ovalbumin Santa Cruz
10D8 IgM O-GlcNAc Santa Cruz Biotechnology BSA-AP- GlcNAc BSA-AP or Ovalbumin Santa Cruz
HGAC 85 IgG GlcNAc Novus Biologicals, Abcam, Pierce, Enzo life sciences, Affinity Bioreagents BSA-AP- GlcNAc, Ovalbumin BSA-AP or BSA (Turner et al. 1990)
HGAC 49 IgM GlcNAc No BSA-AP- GlcNAc, Ovalbumin BSA-AP or BSA (Turner et al. 1990)
HGAC 39 IgG GlcNAc No BSA-AP- GlcNAc, Ovalbumin BSA-AP or BSA (Turner et al. 1990)

BSA-AP, BSA-aminophenol

*

succinylation of WGA (sWGA) reduces it’s affinity for Sialic Acid. Notably, it also reduces the affinity of sWGA for GlcNAc residues. As such, typically one use WGA for purification and sWGA for immunoblotting.