Volume	50 ml	60 ml	10 ml
Acrylamide concentration	10%	12.5%	15%	10%	12.5%	15%	10%	12.5%	15%
g UREA	24	24	24	28.8	28.8	28.8	4.8	4.8	4.8
ml 40% Acryl (29:1)	12.5	15.625	18.75	15	18.75	22.5	2.5	3.125	3.75
ul 30% APS	166	166	166	199.2	199.2	199.2	33	33	33
ul TEMED	20	20	20	24	24	24	4	4	4
10 x TBE	5	5	5	6	6	6	1	1	1
Fill up the volume with deionized, distilled water