Fig. 1.

Immunohistochemistry. a Myocardial tissue samples were labeled with specific antibodies against TnI, the phosphorylated form of TnI (P-TnI) and nuclei with DAPI. Disparate amounts of P-TnI intensities resulted in variable yellow intensities on the merged picture of the HF pacing group. b Capturing images of P-TnI stained slices under a confocal microscope revealed cell-to-cell differences: clusters of cardiomyocytes with divergent levels of TnI phosphorylation could be distinguished in the HF animals at the pacing site