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Characterization of gene disruption mutant ΔP450–1-T35. (A) Total ion currents from GC/MS analyses of extracts of culture filtrates and mycelia from G. fujikuroi strains IMI 58289 (wild type) and ΔP450–1-T35. (B and C) HPLC radiochromatograms of extracts from the culture filtrates and mycelia after incubation of ent-[14C4]kaurenoic acid (B) and [14C4]GA12-aldehyde (culture filtrate only) (C) with IMI58289 and ΔP450–1-T35.
