Table 1.
Products identified by GC/MS from incubations of isotopically labeled intermediates with mutant strain SG139 transformed with P450-1
| Substrate | Product |
|---|---|
| ent-[17-2H2]kaurenoic acid* | ent-7α-hydroxy[2H2]kaurenoic acid |
| [2H2]GA12 | |
| [2H2]GA14 | |
| 7β-hydroxy[2H2]kaurenolide | |
| 7β,18-dihydroxy[2H2]kaurenolide | |
| 3β,7β-dihydroxy[2H2]kaurenolide | |
| [2H2]fujenoic acid | |
| ent-7α-hydroxy-[14C4]kaurenoic acid | [14C4]GA14 |
| ent-6α, 7α-dihydroxy-[14C4]kaurenoic acid* | [14C4]fujenoic acid |
| [14C4]fujenoic triacid | |
| [14C4]GA12-aldehyde | [14C4]GA14 |
| [14C4]GA12 | [14C4]GA14 |
The mass spectrometric data are provided as Table 2, which is published as supplemental data on the PNAS web site, www.pnas.org.
*
Total extracts of culture filtrates were analyzed by GC/MS. Otherwise, extracts were separated by HPLC, and fractions containing radioactivity were analyzed.