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. 2001 Apr 24;98(10):5844–5849. doi: 10.1073/pnas.101126598

Figure 6.

Figure 6

In vitro transcription activation from tox promoters by RNA polymerase core enzyme and TxeR. In vitro transcription reactions were carried out in the presence of E. coli RNA polymerase holoenzyme (H) or core enzyme (C) (A and B) or B. subtilis RNA polymerase holoenzyme (H) or core enzyme (C) (C) with or without TxeR by using as templates DNA fragments containing the promoters of the gdh, toxA, or toxB genes. In lanes marked with an asterisk, contaminating proteins from E. coli not expressing TxeR were added. The arrows indicate the positions of the toxA and toxB transcripts. The sizes of expected gdh transcripts are 122 and 251 nt.