Skip to main content
. 2001 Apr 24;98(10):5844–5849. doi: 10.1073/pnas.101126598

Figure 7.

Figure 7

Interaction of purified TxeR with E. coli RNA polymerase core enzyme. Core RNA polymerase was spotted onto nitrocellulose membranes and immunoblotted by using anti-TxeR antibodies after incubation with crude extract of E. coli (200 μg) carrying the vector pET28-b (filter 1), crude extract of E. coli carrying pCD54 (expressing TxeR) (filters 2–4), or heat-inactivated crude extract of TxeR-expressing cells (filter 5). The triangle indicates the use of increasing amounts of crude extract of E. coli carrying pCD54 (50, 100, and 200 μg).