Figure 1. (A) Structural model of the CAL PDZ domain (green and blue) bound to a CFTR C-terminus mimic (gray) used as input for computational designs (PDB id: 2LOB).

Residues shown in blue were modeled as flexible during the design search. (B) Model of the CFTR trafficking pathway with PDZ domain containing proteins NHERF1 and CAL. CAL is associated with lysosomal degradation of CFTR, while NHERF1 is associated with insertion of CFTR into the cell membrane.