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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: J Mol Cell Cardiol. 2012 Feb 1;52(5):1176–1182. doi: 10.1016/j.yjmcc.2012.01.017

Fig. 3. Effects of Tunicamycin and ATF6 on miR-455 and Calr mRNA Levels in Cultured Cardiac Myocytes.

Fig. 3

Panel A. Neonatal rat ventricular myocyte cultures (NRVMCs) were infected with control adenovirus (Con) or a recombinant adenovirus encoding activated ATF6(1-373) (ATF6) (n = 3 cultures per treatment). One of the control adenovirus-infected sets of cultures was treated with tunicamycin (TM) at 10 μg/ml for 20h. Cultures were then extracted and total RNA was subjected to RT-qPCR for miR-455, and U6 RNA. The mean miR-455/U6 RNA ± S.E. is shown (n = 3 cultures per treatment). *, p < 0.05 different from Con.

Panel B. NRVMCs, treated with TM or ATF6, as described in Panel A, were extracted and total RNA was subjected to RT-qPCR for Calr mRNA and GAPDH mRNA. The mean Calr/GAPDH mRNA ± S.E. is shown (n = 3 cultures per treatment). *, p < 0.05 different from Con.