Table 2. Number of IFNγ+ and Pfn+ cells in heart tissue sections of T. cruzi-infected C57BL/6 mice.
dpi1 | IFNγ+ cells /100 fields2 | Pfn+ cells /100 fields | Ratio of IFNγ+/Pfn+ cells3 |
15 | 50±28 | 0 | 50∶0 |
30 | 54±17 | 5±1 | 11∶1 |
45 | 103±45 | 7±1 | 14∶1 |
60 | 105±16 | 18±3 | 5∶1 |
90 | 92±15 | 18±3 | 5∶1 |
120 | 66±7 | 17±3 | 4∶1 |
dpi, days post-infection with 100 bt of the Colombian T. cruzi strain.
The number of positive cells for each parameter was counted in 100 microscopic fields per section. Three sections were counted per each analyzed animal.
Ratio of IFNγ+/Pfn+ cells was determined adopting the mean number of positive cells for each parameter per 100 microscopic fields.