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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Nat Immunol. 2012 Mar 25;13(5):465–473. doi: 10.1038/ni.2260

Figure 6. Del-1 inhibits IL-17 and periodontal inflammation in old mice.

Figure 6

Eighteen-month-old C57BL/6 mice were microinjected in the gingiva with BSA (control) or Del-1, as indicated. In addition, the mice were orally administered P. gingivalis in 2% carboxy-methylcellulose vehicle (a, b, c; lower rows) or vehicle control (a, b, c; upper rows) and were sacrificed 12h later. Sagittal sections of interdental gingiva were stained for the neutrophil marker Ly6G (a), IL-17A (b), or TNF (c). Shown are typical fluorescent confocal images (left) and their overlays with corresponding DIC images (right). (d) The fluorescence intensities of these and additional representative images from independent mice were quantified using ImageJ analysis; data were expressed as % intensity of the Del-1-treated groups relative to the BSA-treated controls, the value of which was set to 100% (dashed line). `Induced inflammation' refers to the groups inoculated with P. gingivalis. Data are means ± SD (n=5 mice per group) from one of two independent experiments yielding similar results. **P < 0.01 compared to BSA-treated controls.