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. Author manuscript; available in PMC: 2013 Apr 3.
Published in final edited form as: Biochemistry. 2012 Mar 21;51(13):2921–2929. doi: 10.1021/bi3000676

Table 2.

Kinetic parameters for ssDNA-dependent ATP hydrolysis by RecQ.b

Substrate Kact (μM nucleotides) kcat (s−1)
Oligonucleotides (dTn) 0.4±0.2 38±2
Poly dT 0.14±0.02 32±1
M13 ssDNA 0.27±0.04 38±2
Poly dA 0.22±0.03 51±2
b

The parameters values for Kact and kcat were determined from a fit of equation 1 to the data in Figure 2 and Figure 3. For the DNA oligonucleotides, the Kact value reported is the average for all lengths above 12 nucleotides. All values reported are best fit values with the standard deviation from NLLS fitting.