Table 1.
The clathrin-binding motifs.
| adaptor motif | clathrin HC location1 | key clathrin HC residues1 | examples | sequence motif | |
|---|---|---|---|---|---|
| TD site 1: clathrin-box motif | LØXØ[DE] | TD groove between blades 1 & 2 | Ile80 Thr87 Gln89 Phe91 Lys96 Lys98 |
amphiphysin β-arrestin 1 AP-2 β2 subunit S.c.2 Ent1/2p S.c. Yap1802p S.c. Apl2p (AP-1 β1) |
LLDLD LIEFE LLNLD LIDL*4 LIDM* LLDLD |
| TD site 2: W-box motif | PWXXW | top of β-propeller | Phe27 Gln152 Ile154 Ile170 |
amphiphysin SNX9 |
PWDLW PWSAW |
| TD site 3: β-arrestin 1L site | [LI][LI]GXL | TD groove between blades 4 & 5 | R188 Q192 |
β-arrestin 1L AP-2 β2 subunit |
LLGDL LLGDL |
| TD site 4 | unknown | TD exposed surface of blade 7 | Glu11 | unknown | not available |
| ankle | unknown non linear? | CHC1R1/2 | Cys682 Gly710 |
GGA1 | Thr613 + Asn622 |
1
See Figure 1D and 1E for the relative positioning of the four TD interaction surfaces.
2
The locations of the colored side chains are indicated in Figure 1D and 1E.
3
S.c. is Saccharomyces cerevisiae
4
The * symbol indicates the extreme C-terminus of the protein.