Fig. 6. Localization of GS15 to chlamydial inclusion is abrogated in COG3 KD cells.
HeLa cells stably expressing GFP-GS15 (A, B), or YFP-COG3 (C) were treated with control siRNA (A), COG3 siRNA (B) or GS15 siRNA (C) for 72 h and then infected with C. trachomatis serovar D and fixed 24 h post infection. Cells were stained for COG6 (HiLyte Fluor-555) and for DAPI (A,B) or IncA (HiLyte Fluor-488) (C). The merge images are shown on the right. Arrows indicate the membrane of chlamydial inclusions. Scale bar, 10 µm.