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A phoP∷Tn10 mutant killed fewer U-937 macrophages than wild-type S. typhimurium by 4 hr after infection. (A) Suspended and adherent cells were harvested and processed for FACS. The percentage of TUNEL-positive events is shown. (B) Adherent U-937s were TUNEL and DAPI stained for fluorescence microscopy. U, uninfected; WT, wild-type; phoP, phoP∷Tn10 mutant; orgA, orgA∷Tn10 mutant.
