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. Author manuscript; available in PMC: 2012 Apr 20.
Published in final edited form as: Oncogene. 2010 Nov 15;30(10):1229–1240. doi: 10.1038/onc.2010.504

Figure 2.

Figure 2

YAP1 enhanced migration and invasion abilities of MIHA cells. (a) Wound healing assay. At 48 h, both MIHA-YAP1a and MIHA-YAP1b clones exhibited faster closure of the gap than MIHA-Vec cells did. Images were taken immediately after scratching the cultures 0 h, 24 h and 48 h later. Magnification: × 200, scale bar: 40 µm. (b) Matrigel invasion assay. Representative fields of invaded cells on the membrane are shown. Penetrated cells were stained with 0.1% crystal violet. MIHA-YAP1 clones were able to penetrate the Matrigel membrane after 72-h incubation. Histograms in the lower panel showed the mean cell counts of the invaded cells. Magnification × 100, scale bar: 80 µm; magnification: × 400, scale bar: 20 µm.