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. 2011 Aug 29;31(16):2062–2074. doi: 10.1038/onc.2011.383

Figure 6.

Figure 6

CpG island hypermethylation-associated silencing of miR-200c141 in human colorectal cancer. (a) Isolation of epithelial and stromal cells from normal and tumor colonic mucosa using laser microbeam microdissection and pressure catapulting. (b) Aberrant miR-200c141 CpG island hypermethylation in tumor epithelial cells (TE) in comparison with corresponding unmethylated normal epithelial crypts (NE), evaluated by bisulfite genomic sequencing. Both normal stroma (NS) and tumor stroma (TS) samples shown dense methylation. (c) Decreased expression of mature miR-200c and miR-141 in hypermethylated TE samples in contrast to high expression in paired normal epithelium samples. Both NS and TS samples showed low levels of miR-200c141. Data represent mean±s.d., obtained by qRT–PCR (Student's t-test P<0.05).