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. 2011 Sep 26;31(16):2075–2089. doi: 10.1038/onc.2011.396

Figure 2.

Figure 2

Supplementing bioactive lipids LPA and S1P to CSS supports acinar differentiation and represses invasive transformation. (a) Concentrations of 1  and 10 μ LPA effectively repress invasive properties in lrECM for 36 and 42 h, respectively, maintaining the round phenotype. (b) S1P concentrations of 1  and 10 μ in CSS repress invasiveness for close to 100 h. (c) Immunofluorescent images of PC-3 spheroids stained for filamentous actin (F-actin) and laminin α-1 demonstrate disintegration of the actin cytoskeleton and first indication of the beginning disintegration of the BM after 24 h exposure to 10% lipid-deprived CSS versus complete medium. In CSS supplemented with 10 μ LPA or 1 μ S1P, both actin cytoskeleton and BM remain intact.