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. 2011 Sep 26;31(16):2075–2089. doi: 10.1038/onc.2011.396

Figure 6.

Figure 6

Cell line-specific effects of ROCK inhibitors and increased cAMP levels on acinar morphogenesis versus formation of invasive structures. Spheroids formed by PC-3, LNCaP and EP156T cells in Matrigel were stained with Alexa488-phalloidin for filamentous F-actin, and an antibody against human laminin-α1 to highlight the BM. Inhibition of ROCK kinases (Y-27632) and enhanced AC/increased cAMP levels (forskolin) induce invasive features and disintegration of the BM in transformed PC-3 and LNCaP spheroids, but promote maturation of non-transformed EP156T cells.