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. Author manuscript; available in PMC: 2012 Apr 20.
Published in final edited form as: Dev Biol. 2007 Feb 21;305(1):347–357. doi: 10.1016/j.ydbio.2007.02.022

Figure 2. cdc-42(RNAi) results in multiple ACs, adjacent 1° VPC fates and gonad splitting.

Figure 2

Overlays of Nomarski images of L3 Pn.pxx stage animals with projections of zmp-1::YFP expression (yellow) marking the Anchor Cell (AC) and egl-17::CFP expression (blue) marking 1° fate VPCs. Brackets indicate the descendants of induced VPCs and black dotted outlines the morphology of the gonad.

(A) wild-type with extended gonad (inset), a single AC, and three induced VPCs, one of which (P6.p) adopts the 1° fate (marked by egl-17::CFP).

(B-D) cdc-42(RNAi) animals with (B) two touching ACs and a single 1° fate VPC, (C) two ACs in separate gonad fragments and two, non-adjacent, 1° fate VPCs, (D) two separated ACs in an intact, but short, gonad and two adjacent 1° fate VPCs. . VPCs adjacent to 1° fate VPCs sometimes fail to adopt vulval fates (arrows in D), perhaps because of a gap between the VPCs.

Anterior, left; ventral, down; scale bar, 10μm

(E) Table of 1° vulval fate phenotypes

11° fate scored using arIs92[egl-17::CFP::lacZ] (Yoo et al., 2004)

21° fate scored using syIs59[egl-17:CFP] (Inoue et al., 2002)

AC: Anchor Cell