Table 1.
VPCs are hyperinduced following RNAi of cdc-42, par-3, par-6 or pkc-3
| Treatment | Length of Exposure (hours) |
% animals hypo- induced |
% animals hyper- induced |
Average Induction (Range) |
n | % Muv3 |
n |
|---|---|---|---|---|---|---|---|
| Vector(RNAi) | 48-72 | 0 | 0 | 3.01 (3.0-3.0) | 30 | 0 | 1793 |
| cdc-42(RNAi) | 48-72 | 10 | 47 | 3.41 (2.0-5.0) | 30 | 28 | 1971 |
| par-3(RNAi) | 48-72 | 0 | 10 | 3.11 (3.0-5.0) | 30 | 7 | 188 |
| par-6(RNAi) | 24-48 | 0 | 10 | 3.21 (3.0-6.0) | 30 | 4 | 233 |
| pkc-3(RNAi) | 48-72 | 0 | 13 | 3.11 (3.0-4.5) | 30 | 6 | 151 |
| mes-1(RNAi) | 40-644 | n.d. | n.d. | n.d. n.d. | n.a. | 75 | 206 |
|
| |||||||
| cdc-42(RNAi) 1 AC | 48-72 | 4 | 4 | 3.02 (1.0-4.0) | 55 | n.d. | n.a. |
| cdc-42(RNAi) 2+ AC | 48-72 | 4 | 80 | 4.12 (2.0-6.0) | 45 | n.d. | n.a. |
1
The number of VPCs induced (from 0-6) was scored by counting vulval cells at L4 Pn.pxx.
2
The number of VPCs induced (from 0-6) was scored by counting vulval cells at L3 Pn.pxx.
Greater than 3 induced VPCs is hyperinduced and fewer than 3 is hypoinduced. Hypoinduction may be a result of gaps between VPCs or abnormal AC positions.
3
Muv, multiple protrusions scored using a dissecting microscope.
4
mes-1(RNAi) incubations were at 25°C.
5
previously reported by Capowski et al (1991)
AC: Anchor Cell