Skip to main content
. 2001 Apr 24;98(10):5880–5885. doi: 10.1073/pnas.101524998

Figure 3.

Figure 3

A PTP underlies the effect of IGF-I on rod CNG channels. (A) Application of a tyrosine kinase inhibitor “traps” channels in a modulated state. Rods were pretreated with IGF-I (100 nM) for the entire 10 min, for the first 5 min with a 5-min wash with control saline, or for 5 min with a 5-min wash with Lav A (10 μM). As a control, rods were pretreated with Lav A alone for 5 min before seal formation. (B) An exogenous PTP increases the cGMP sensitivity of rod CNG channels. Direct application of TC PTP (1–4 units/100 μl) on inside-out patches from control and IGF-I-pretreated (1 μM for 15 min) rods. Patches were exposed to TC PTP for 5 min before determination of the K1/2 for 8-Br-cGMP. Vanadate (100 μM) partly inhibited the effect of TC PTP (n = 6–11).