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ERK facilitates ATR S428 phosphorylation in response to HU. A) MCF7 cells were stably infected with empty vector (Ctrl), ERK1 shRNA, and ERK2 shRNA. The expression of ERK1, ERK2, and actin was examined by western blot using the specific antibodies. B) MCF7 Ctrl (control), ERK1 shRNA and ERK2 shRNA cells were treated with HU at the indicated doses for 24 hours, followed by analysis of ATR S428 phosphorylation (Phos-S428 ATR) (Cell Signaling, 1:1000) and total ATR (Calbiochem, 1:1000).
