Table 1.
Experimental diets
| Ingredient | Casein | GMP | Amino Acid |
|---|---|---|---|
| Protein | |||
| Casein | 200 | ||
| BioPure GMP* | 200 | ||
| l-Arginine HCl | 4.6 | 12.1† | |
| l-Cystine | 3.0 | 3.5 | |
| l-Histidine, HCl-H2O | 3.2 | 4.5 | |
| l-Leucine | 6.8 | 11.1 | |
| l-Methionine | 7.0 | 8.2 | |
| l-Phenylalanine | 1.64 | 2.5 | |
| l-Tyrosine | 5.0 | 5.0 | |
| l-Tryptophan | 1.3 | 1.8 | |
| Carbohydrate | |||
| Sucrose | 250 | 250 | 250 |
| Cornstarch | 241 | 225 | 268 |
| Maltodextrin | 130 | 130 | 130 |
| Cellulose | 50 | 50 | 50 |
| Fat | |||
| Soybean oil | 70 | 70 | 70 |
| Choline bitartrate | 2.5 | 2.5 | 2.5 |
| Vitamins and Minerals | |||
| Vitamin mix AIN-93-VX | 13 | 13 | 13 |
| Mineral mix, 98057‡ | 35 | 35 | 35 |
| Sodium chloride | 4.2 | 3.6 | |
| Sodium phosphate, dibasic | 7.2 | 8.0 | |
| Potassium phosphate, dibasic | 2.0 | 2.0 | |
| Calcium phosphate, dibasic | 5.25 | ||
| Calcium phosphate, monobasic | 9.5 | ||
| Calcium carbonate | 13.7 | 6.75 | 9.9 |
| Antioxidant | |||
| t-Butylhydroquinone | 0.014 | 0.014 | 0.014 |
Values are in g/kg. GMP, glycomacropeptide.
*
BioPURE GMP; Davisco Foods International, Inc., LeSueur, MN.
†
In addition, the following l-amino acids were included for a total of 175 g amino acids/kg diet: alanine, 3.5; asparagine, 6.0; aspartic acid, 3.5; glutamic acid, 40; glycine, 23.3; isoleucine, 8.2; lysine HCl, 18.0; proline, 3.5; serine, 3.5; threonine, 8.2; and valine, 8.2, based on Rogers and Harper (48).
‡
Teklad Custom Diets, Madison, WI; mineral mix without calcium and phosphorus.