Fig. 2.

Glucose tolerance and utilization in HA-GLUT4-GFP transgenic mice. A: insulin tolerance test performed in nonfasted mice with injection of insulin (0.75 IU/kg ip). Data are means ± SE; n = 5. B: glucose tolerance test performed using mice fasted overnight. Glucose was injected (2 g/kg ip). Data are means ± SE; n = 6. C: insulin-dependent glucose uptake in isolated extensor digitorum longus (EDL) muscles from WT C57BL/6J and Tg mice (HA-GLUT4-GFP). Basal and insulin-stimulated glucose uptake was measured using 2-deoxy-[¹⁴C]glucose. Data are means ± SE; n = 3. D: insulin-dependent glucose uptake in soleus muscles. Soleus muscles were isolated from WT C57BL/6J and Tg mice (HA-GLUT4-GFP). Basal and insulin-stimulated glucose uptake was measured using 2-deoxy-[¹⁴C]glucose. Data are means ± SE; n = 3.