Fig. 4.

Dependency of Ang III and Ang-(1–7) formation on incubation time and Ang II substrate concentrations. Kidney sections were incubated with varying concentrations of Ang II (10–1,000 μmol/l) for 5 and 15 min. Metabolism of Ang II and formation of Ang III/Ang-(1–7) peptides were analyzed by MALDI imaging. A: presence of Ang II after incubation of kidney sections with 10–1,000 μmol/l Ang II for 5 and 15 min. B: formation of Ang III after 5 and 15 min of incubation with 10–1,000 μmol/l Ang II. C: formation of Ang-(1–7) after 5 and 15 min of incubation with 10–1,000 μmol/l Ang II. Note that bars are absent for 10, 50, and 100 μmol/l in A and C.