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Theoretical and observed ion mass for Ang II metabolic assay
| Ang Species | Amino Acid Sequence | Theoretical Mass (m/z) | Observed Mass (m/z) |
|---|---|---|---|
| Ang II | DRVYIHPF | 1,046.2 | 1,046.2 |
| Ang III | RVYIHPF | 931.1 | 931.0 |
| Ang-(1–7) | DRVYIHP | 899.0 | 899.0 |
| Ang IV | VYIHPF | 774.9 | <LOD |
| Ang-(1–5) | DRVYI | 664.8 | <LOD |
| Ang-(1–4) | DRVY | 551.6 | 551.6 |
| Ang-(5–8) | IHPF | 512.6 | <LOD |
Ang, angiotensin; m/z, mass-to-charge ratio; LOD, limit of detection (∼5 ng).
