Fig. 5.

PMN-mediated melanoma adhesion in the presence of sFn is dependent on a divalent binding mechanism. A: fibrin(ogen) fragment D failed to mediate PMN-facilitated 1205Lu adhesion to EC. B: thrombin and fibrinogen concentrations affect melanoma cell adhesion. sFn-high, sFn-med, sFn-low were made, respectively, from 1.5 mg/ml sFg + 2 U/ml thrombin; 1.5 mg/ml sFg + 0.053 U/ml thrombin; and 0.015 mg/ml sFg +2 /ml thrombin. sFg was 1.5 mg/ml sFg without adding thrombin. *P < 0.05 compared with the case sFn (A) or sFn-high (B), respectively, in the same arrest duration. Values are means ± SE for n ≥ 3.