Fig. 8.

Schematic diagram of receptor-ligand pairings with sFn as a cross-linking molecule. When ICAM and α_Vβ₃ bearing melanoma cells approach ICAM-1 bearing ECs and Mac-1 bearing PMNs, 4 types of bonds (ICAM-1)_TC-(sFn)-(ICAM-1)_EC, (α_vβ₃)_TC-(sFn)-(ICAM-1)_EC, (ICAM-1)_TC-(sFn)-(Mac-1)_PMN, and (α_vβ₃)_TC-(sFn)-(Mac-1)_PMN via freely flowing sFn molecules could form. ICAM-1-mediated bonds initiated short-term tethering, while α_vβ₃ -mediated bonds were responsible for firm adhesion of melanoma cells to either EC or PMN.