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Actin depolymerization abrogates UT-A1 endocytosis. A: cell biotinylation experiment. UT-A1-MDCK cells were treated with 5 mM methyl-β-cyclodextrin (MCD) or 50 μg/ml concanavalin A (Con A) together with or without 1 μM latrunculin B for 1 h. Cells were then processed for biotinylation. Total cell lysates or biotinylated proteins were analyzed for UT-A1 expression. B: quantification of cell surface UT-A1 from 3 independent experiments (means ± SD; **P < 0.01).
