Fig. 5.

Akt phosphorylation stimulated by ET-1 and PDGF is not reduced in Abl knockdown cells. UI cells, cells expressing control shRNA, and stable Abl knockdown cells were treated with 200 nM ET-1 (A) or 20 ng/ml PDGF (B) for 10 min. Akt phosphorylation (Ser⁴⁷³) induced by ET-1 and PDGF was similar in UI cells, cells treated with control shRNA, and Abl knockdown cells (P > 0.05). Stimulated Akt phosphorylation (p-Akt) was normalized to the basal level of uninfected cells. Values are means ± SE (n = 5–6).