| Component | Amount (μl) |
| Cy3-labeled der A and Cy5-labeled der B | 158 |
| Cot-1 DNA (1 mg ml − 1) | 50 |
| Agilent 10× Blocking Agent | 52 |
| Agilent 2× hybridization buffer | 260 |
| Total volume | 520 |
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