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A: actual tracings show the development of spontaneous and steady basal tone in the smooth muscles of the human internal anal sphincter (IAS) vs. rectal smooth muscle (RSM); the latter primarily has phasic activity. B: bar graph data show significantly higher basal tone in the IAS vs. the RSM (*P < 0.05; n = 6–8). C: quantitative data show that electrical field stimulation (EFS) causes frequency-dependent relaxation of the IAS that is obliterated by the neurotoxin TTX (10−6 M).
