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Protein gene product (PGP)9.5 immunostaining of the distal colorectum. A–C: PGP9.5-immunoreactive nerve fibers in A were quantified as areas (proportions) with respect to the colorectal mucosal and submucosal area (B), which were normalized by control mean PGP9.5 proportions (C). Intracolonic treatment with Zym did not affect the density of PGP9.5 immunoreactivity.
