Table 1.
Primer | Sequence |
---|---|
B2m | Forward: 5′-TGC TAC GTG TCT CAG TTC CA-3′ |
Reverse: 5′-GCT CCT TCA GAG TGA CGT GT-3′ | |
TG1 | Forward: 5′-GAC TAC TCT CGA GGC ACC AA-3′ |
Reverse: 5′-CGT GTG CAG AGT TGA AGT TG-3′ | |
TG2 | Forward: 5′-GGG AAT ACG TCC TCA CAC AG-3′ |
Reverse: 5′-GTC ATC ATT GCA GTT GAC CA-3′ | |
TG3 | Forward: 5′-GAA CCT GGA ACG GTA GTG TG-3′ |
Reverse: 5′-GCT ATC ACT GCC TTT CTC CA-3′ | |
TG4 | Forward: 5′-ATA GAA TGC ACC CCA GTG AA-3′ |
Reverse: 5′-ACA TGC TTA CCA AGG CTC AG-3′ | |
TG5 | Forward: 5′-TAT TTT CAA ACC CCC TCT CG-3′ |
Reverse: 5′-TCT GCC TTT GTC CAC TCT TG-3′ | |
TG7 | Forward: 5′-GGA CAG CCT GTG AAA TAT GG-3′ |
Reverse: 5′-GGT GGA AGG TCT TTC CTG AT-3′ | |
FXIII | Forward: 5′-AAA CTG CCC TGA TGT ATG GA-3′ |
Reverse: 5′-CCC CAG TGT AGA AGG TGA TG-3′ |
Forward and reverse sequence of the primers used for RT-PCR. The uniqueness of each of the primers was confirmed by a BLAST search using each primer set biased toward picking “somewhat dissimilar sequences.” B2m, β2-microglobulin; TG, transglutaminase; FXIII, Factor XIII.