Table 2.
TG mRNA expression
| ΔCt |
||
|---|---|---|
| Aorta | Vena Cava | |
| TG1 | 0.15 ± 0.03 | 2.0 ± 0.6* |
| TG2 | 2 ± 1 | 12 ± 3* |
| TG3 | ND | ND |
| TG4 | 2.0 ± 0.5 | 2.4 ± 0.7 |
| TG5 | ND | ND |
| TG7 | 0.02 ± 0.01 | 0.03 ± 0.02 |
| FXIII | 3.7 ± 0.2 | 4 ± 1 |
Values are means ± SE; n = 3. ΔCt, change in threshold cycle. For simplicity, values have been multiplied by 1,000. Primers targeted to rat TG1 through TG5, TG7, and FXIII were used to measure mRNA expression in ∼10 mg of aorta or vena cava tissues. mRNAs to 4 of the TGs were found in both tissues: TG1, TG2, TG4, and FXIII. Negligible amounts of mRNA were also found for TG7. Expression of the TGs is expressed relative to B2m. ND, no detection of mRNA by RT-PCR analysis.
*
P ≤ 0.05, significantly different expression from aorta.