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. 2012 Apr 19;7(4):e34949. doi: 10.1371/journal.pone.0034949

Figure 4. Alteration of nuclear long ncRNA expression by anticancer agents in HeLa Tet-off (TO) cells.

Figure 4

(A, B) HeLa Tet-off (TO) cells were treated with 20 µg/ml MMC (A), and 5 or 20 µg/ml MMC (B) and subjected to qRT-PCR. RNA levels were normalized to those of β-actin, and are presented relative to non-treated cells. GADD45a was used as a positive control induced by MMC. (C, D) HeLa Tet-off (TO) cells were treated with 1.0 nM DOX (C) and 0.5 or 1.0 nM DOX (D) and subjected to qRT-PCR. RNA levels were normalized to those of GAPDH, and are presented relative to non-treated cells (con). p21 was used as a positive control induced by DOX. Error bars show the experimental error of two experiments.