Skip to main content
. 2012 Apr 19;7(4):e35674. doi: 10.1371/journal.pone.0035674

Figure 2. ANX7 suppresses cell motility and invasion in PDAC cells.

Figure 2

A. Negative scrambled control (Scr-1) and BART RNAi (siBART-1) S2-013 cells in confluent cultures were wounded. After 4 h, the cells were immunostained using anti-BART (green) and anti-ANX7 (red) antibodies. Blue, DAPI staining. Arrows, colocalized BART and ANX7 at the leading edge of control cells. Bars, 10 µm. B. siRNA oligonucleotides targeting ANX7 (siANX7) and negative scrambled control were transiently transfected into S2-013 and PANC-1 cells. Western blotting validated ANX7 knockdown in both cell lines. C. Transwell motility assay of cells treated as in (B). Migrated cells in four fields per group were counted. Data are representative of three independent experiments. Columns, mean; bars, SD. *p<0.005 compared with control cells. D. Quantification of the two-chamber invasion assay of cells treated as in (B). Invaded cells in four fields per group were counted. Data are representative of three independent experiments. Columns, mean; bars, SD. *p<0.001 compared with control cells.