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. 2012 Apr 19;7(4):e35674. doi: 10.1371/journal.pone.0035674

Figure 3. ANX7 and phosphorylated PKC are colocalized at the leading edges of migrating cells.

Figure 3

A. Immunoprecipitation of endogenous ANX7 or PKC from S2-013 cells. Immunoprecipitates were examined by Western blotting using anti-ANX7 and anti-PKC antibodies. Normal mouse or rabbit IgG was used as the isotype control for ANX7 or PKC, respectively. B. Western blot with anti-PKC and anti-phospho-PKC antibodies showing S2-013 cells transiently transfected with siRNA for ANX7 as compared to cells transfected with scrambled control. C. Immunocytochemical staining in S2-013 cells, as determined with anti-ANX7 (green) and anti-phospho-PKC (red) antibodies. Blue, DAPI staining. Arrows, colocalized ANX7 and phosphorylated PKC at lamellipodial-like protrusions. Bar, 10 µm. D. Confluent S2-013 cells were wounded. After 4 h, the cells were immunostained using anti-ANX7 (green) and anti-phospho-PKC (red) antibodies. Blue, DAPI staining. Arrows, colocalized ANX7 and phosphorylated PKC at the leading edge. Bar, 10 µm.