Figure 6. PKC phosphorylation is not directly regulated by ANX7.
A. S2-013 and PANC-1 cells were prelabeled with [32P]-orthophosphoric acid and stimulated or not with PMA. ANX7 was immunoprecipitated, separated by SDS-PAGE, and analyzed by autoradiography. An immunoblot was performed on the cell lysate as a loading control. B. In vitro phosphorylation assays to investigate the effect of BART and ANX7 on PKC-phosphorylation. Purified rat brain was incubated with recombinant ANX7 with or without recombinant BART. The reaction products were analyzed by an ELISA assay. ABS on Y-axis means absorbance at 492 nm as reference measured with a microplate reader.