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. 2012 Apr 19;7(4):e35674. doi: 10.1371/journal.pone.0035674

Figure 9. PKCα is associated with increased cell invasion by knockdown of BART and ANX7.

Figure 9

A. BART RNAi S2-013 cells and S2-013 cells transiently transfected with ANX7-siRNA were pretreated with or without Ro-32-0432. The activity of PKCα and η was assessed by Western blotting. B. Cells shown in (A) were pretreated with or without safingol. The activity of PKCα and PKCη was assessed by Western blotting. C. The effect of Ro-32-0432 on cell invasion was investigated using the transwell invasion assay. Migrated cells in four fields per group were counted. Data are representative of three independent experiments. Columns, mean; bars, SD. *p<0.001 compared with non-treated cells. D. The effect of safingol on cell invasion was investigated using the transwell invasion assay. Data are representative of three independent experiments. Columns, mean; bars, SD. *p<0.001; **p<0.003 compared with non-treated cells. E. The effect of rottlerin and pseudosubstrate PKCη inhibitor on cell invasion was investigated using the transwell invasion assay. Data are representative of three independent experiments. Columns, mean; bars, SD.