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. 2012 Jan 25;7(4):761–769. doi: 10.1021/cb200450w

Figure 2.

Figure 2

FA assay of CP binding to WM CA-CTD and GagΔp6. (a) Plots of FA as a function of WM CA-CTD concentration for fluorescein-labeled CP2 and CP4 (100 nM each). (b) The binding of GagΔp6 to fluorescein-labeled CP1, CP2, and CP4 (100 nM each). (c) Competition between fluorescein-labeled CP2 (100 nM) and unlabeled CP2 and its Asn-scan variants (0–10 μM) for binding to WM CA-CTD (2 μM) (Supporting Table s4). (d) Competition between fluorescein-labeled CP4 (100 nM) and unlabeled CP4 and its Asn-scan variants (0–10 μM) for binding to WM CA-CTD (2 μM).