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Structures of (a) CP2 and (b) CP4. (c) FA assay to determine the binding stoichiometry of CP2 and CP4 to WM CA-CTD. Increasing WM CA-CTD was added to a mixture of 10 μM fluorescein-labeled CP2 or CP4 and 590 μM unlabeled CP2 or CP4 in binding buffer (see Methods). Binding stoichiometry was obtained by determining the intersection of the linear fits to the first 5 data points and the last 4 data points.
