Figure 3.

Cloning and analysis of the DGTS operon of R. sphaeroides. (A) Partial restriction map of cosmid pRKL301 complementing RKL3 and complementing subclones of pRKL301. The region of pRKL301 common to all subclones is indicated by solid line. B, BamHI; P, PstI; S. SalI. (B) Structure of the insert of pRKL323 derived from sequence analysis. The two ORFs associated with DGTS biosynthesis, btaA and btaB, are drawn as gray and open boxes, respectively. In addition, a putative promoter (filled box) with consensus sequences (bold) and a putative proline-tRNA gene (Pro-tRNA) are indicated. Three potential start codons (bold) and ribosome binding sites (bold) are shown. Numbers refer to nucleotides of the sequence deposited at GenBank (accession no. AF329857).