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. 2001 May 1;98(10):5910–5915. doi: 10.1073/pnas.101037998

Figure 5.

Figure 5

Disruption of the btaB gene. (A) Maps of the inactivation construct pbtaB-dis and the genomic DNA surrounding the inactivated btaB gene in R. sphaeroides btaB-dis. The kanamycine resistance cassette is drawn as a black box. Numbers refer to nucleotides of the btaB ORF (open box). (B) Genomic DNA blot of R. sphaeroides and btaB-dis strain probed with the btaB wild-type gene. Genomic DNA was digested with BglII and HinDIII. The blot was probed with the insert of pbtaB. Diagnostic hybridizing fragments visualized by exposure to x-ray film and their approximate length are indicated.