Table 1.
Description of strains and plasmids used in this study
| Strain or plasmid | Description or construction | Source or ref. no. |
|---|---|---|
| R. sp. 2.4.1 | Wild type | ATCC 17023 |
| R. sp. RKL3 | DGTS-deficient btaA MNNG-induced mutant | This study |
| R. sp. btaB-dis | DGTS-deficient btaB disruption mutant | This study |
| E. coli HB101 | F− Δ(mcrC-mrr) leu supE44 ara14 galK2 lacY proA2 rpsL20 (Strr) xyl-5 mtl-1 recA13 | 30 |
| E. coli XL-10 Gold | TetrΔ(mcrA)183 Δ(mcrCB-hsdSMR-mrr) 173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Hte [F′ proAB lacIqZDM15 Tn10 (Tetr) Amy Camr] | Stratagene |
| E. coli DH10B | F−mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZDM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara, leu)7697 galU galKl-rpsL nupG | GIBCO/BRL |
| E. coli MM294 | F−endA1 hsdR17
(r m ) supE44
thi-1 relA1
|
29 |
| pBluescript II SK(+) | Ampr | Stratagene |
| pRK2013 | Kanr Tra+ RK2-ColE1rep | 31 |
| pCHB500 | Tcr; expression vector for R. sphaeroides | 26 |
| pUC4K | Kanr Nmr; contains neomycin phosphotransferase gene of Tn903 | Pharmacia |
| pSUP202 | Ampr Cmr Tcr pBR325rep | 32 |
| pRKL301 | Cosmid clone complementing RKL3 | This study |
| pRKL323 | Smallest subclone of pRKL301 complementing RKL3 | This study |
| pbtaA | Nucleotides 436–1834 of pRKL323 in pCHB500 | This study |
| pbtaANT | Nucleotides 633–1834 of pRKL323 in pCHB500 | This study |
| pbtaB | Nucleotides 1814–2625 of pRKL323 in pBS II SK(+) | This study |
| pbtaB-dis | btaB inactivation cassette in pSUP202 | This study |