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Elution positions of cis-zeatin standard and products of the enzymatic reaction after HPLC as reported (22). (A) cis-zeatin standard. (B) Product of enzymatic reaction after incubating recombinant protein with cis-[14C]zeatin (45,000 cpm) and UDP-glucose (4 mM) in 0.17 M Tris, pH 8.0. (C) Reaction product obtained from B treated with β-glucosidase for 2 h as reported (30), resulting in formation of cis-zeatin.
