Fig. 2.

Allele-specific nuclear protein complex formation of the MMP7 promoter. A: schematic of the known transcription factor binding sites for the proximal MMP7 promoter. B: allele-specific nuclear protein complex formation of the rs11568818AG SNP. EMSA were performed using nuclear proteins isolated from HT-29 cells and oligonucleotides specific for the rs11568818A and G alleles. Competitors (CPT) of unlabeled probes at excess of 80-fold concentration compared with the labeled probes were included in the reactions for self competition and reciprocal competition of the 2 alleles. The nuclear protein complexes specific for A and G allele are labeled as A and G, respectively.