Fig. 8.
Induction of ferritin by heme-released iron is increased in hyperoxic WT mice. Representative Western blot of ferritin and lactoferrin proteins in whole lungs of neonatal mice from WT and HO-1 TG groups following 2 wk of room air (21% O2) or hyperoxia (75%O2) after birth. Quantification of the bands by densitometry revealed a significant increase in the ratio of ferritin to lactoferrin levels in WT hyperoxic mice compared with normoxia or to the TG group. Forty- to fifty-microliter aliquots of lung homogenates were electrophoresed and immunoblotted with anti-ferritin or anti-lactoferrin (see materials and methods). ***P < 0.001 vs. WT-21% O2; #P < 0.05 vs. WT-75% O2.